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C12 NBD ceramide (d18:1/12:0) is a long-chain fluorescent ceramide analog that can be used for the measurement of alkaline and neutral ceramidase activity.{14063} It is hydrolyzed efficiently by alkaline ceramidase from P. aeruginosa and by neutral ceramidase from mouse liver. In addition, alkaline and neutral ceramidases from a variety of mammalian cell lines also hydrolyze C12 NBD ceramide (d18:1/12:0). Acid ceramidase does not appear to utilize C12 NBD ceramide (d18:1/12:0) as a substrate. The possibility exists forC12 NBD ceramide (d18:1/12:0) to also be applied to the measurement of ceramide kinase and/or sphingomyelin synthase activity.
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Polyoxyl 40 stearate is a polyethylene glycol monostearate (PEG-40 stearate) used as a nonionic surfactant and pharmaceutical excipient. Supplied as a white to off-white solid for formulation and analytical use, the material is typically reported with high purity and is used to aid emulsification and solubilization of lipophilic ingredients.
Used as a nonionic emulsifier and surfactant.
Suitable for pharmaceutical formulation and analytical use.
Improves solubility and dispersion of lipophilic compounds.
Available in small laboratory pack sizes for research use.
High reported purity supports analytical applications.
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